BIOCHEMICAL INDICES OF PROOXIDANT-ANTIOXIDANT PROCESSES IN CALENDULA OFFICINALIS L., GROWN UNDER THE INFLUENCE OF GROWTH BIOSTIMULANTS
The effect of plant growth biostimulants «Vermymag», «Vermyiodis» and «Vermystym» on the content of enzymes of antioxidant protection and metabolites of the prooxidant-antioxidant system of C. officinalis flowers of the cultivated variety «Poliova Krasunia» cultivated in the soil and climatic conditions of the Precarpathian region has been analyzed. It was shown that applying biostimulants during cultivation of C. officinalis flowers contributed to the increase (p<0.05-0.01) of antioxidant enzymes activity compared with control plants. In particular, the superoxide dismutase and catalase activity was higher with applying biostimulants «Vermymag» and «Vermyiodis». The biostimulant «Vermymag» had a positive influence on the peroxidase activity, contributing to the increase of enzyme content up to 21% comparing with the control. The content of ascorbic acid increased under the influence of all biostimulants in plant cells. The use of stimulants positively influenced the redox state of C. officinalis cells, contributing to suppressing lipoperoxidation processes and increasing the resistance of plants to the action of stress factors. It is found out that growth biostimulants promote the balance of prooxidant-antioxidant processes in C. officinalis plants.
Bilchuk, V., Rossihina-Galicha, A. (2012). The content of ascorbic acid and activity of enzymes of its metabolism at action of nickel ions in maize seedlings. Visnyk of the Lviv University, series BIology, 60, 332-337. [in Ukrainaian].
Bredford, W. (1976). A simple method for protein test. Annal. Biochem, 72, 248-252. [in English].
Chevari, S., Chaba, I., Sekey, Y. (1985). The role of superoxide dismutase in oxidative processes and the method of its determination in biological materials. Laboratory work, 11, 678-681. [in Russian].
Doliba, I. M., Volkov, R. A., Panchuk, I. I. (2010). Method of catalase activity determination in plants. Physiology and biochemistry of cultivated plants, 42 (6), 497-503. [in Ukrainaian].
Horemans, N., Foyer, C. H., Potters, G. (2000). Ascorbate function and associated transport systems in plants. Plant Physiol. Biochem, 38, 531-540. [in English].
Kolupaev, Yu. E., Karpets, Yu. V., Yastreb, Т. О. (2017). Functioning of plants antioxidative system under salt stress. Visnyk of Kharkiv National Agrarian University, series Biology, 3 (42), 23-45. [in Russian].
Lupak, O., Klepach, H., Antonyak, H. (2018). Marigold (Calendula officinalis L.) and its components as a source of biologically active substances, in: Krynski, A., Tebug, G. K., Voloshanska, S. (Eds.). Ecology and human health. Czestochowa: Educator, 65-76. [in English].
Lupak, O., Kovalchuk, H., Antonyak, H. (2017). Potentiometric determination of antioxidant activity of extracts of Calendula officinalis L. plants under the influence of growth biostimulants. Scientific Journal «Science Rise: Biological Science», 6 (9), 10-13. DOI: 10.15587/2519-8025.2017.119086. [in Ukrainaian].
Makogonenko, S. Yu, Baranov, V. I., Terek, O. I. (2018). The influence of the Regoplant and Stimpo on the activity of antioxidant protection enthymes in the Helianthus annuus L. and Brassica napus L. growth on the substantions of the wet recovery of coal mine. Studia Biologica, 12 (1), 47-57. DOI: 10.30970/sbi.1201.539. [in Ukrainaian].
Mogharrab, N., Ghourchian, H., Amininasaby, M. (2007). Structural Stabilization and Functional Improvement of Horseradish Peroxidase upon Modification of Accessible Lysines: Experiments and Simulation. Biophysical Journal, 92, 1192-1203. [in English].
Musiienko, M. M., Parshykova, T. V., Slavnyi, P. S. (2001). Spectrophotometric methods in phisiological, biochemical and plant ecology practice. Kyiv: Fitosotsiotsentr, 127-129. [in Ukrainaian].
Nasrabadi, H. (2008). Some biochemical properties of catalase from Kohlrabi. Journal of Biological Sciences, 8 (3), 649-653. DOI: 10.3923 / jbs.2008.649.653. [in English].
Terek, О. I. (2018). Mechanisms of plant adaptation to oil pollution. Studia Biologica, 12 (3-4), 141-164. DOI: 10.30970/sbi.1203.579 [in Ukrainaian].
Zhang,Y., Li, Z., Yan Peng,Y, Wang, X., Peng, D., Li, Y., He X.et al. (2015). Clones of FeSOD, MDHAR, DHAR Genes from White Clover and Gene Expression Analysis of ROS-Scavenging Enzymes during Abiotic Stress and Hormone Treatments. Molecules, 20, 20939–20954. DOI:10.3390/molecules201119741. [in English].
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